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Rev. bras. ciênc. vet ; 24(2): 99-103, abr.-jun./2017. il.
Article in Portuguese | LILACS | ID: biblio-966774

ABSTRACT

A pesquisa teve por objetivos avaliar a biometria testicular, coletar o sêmen e analisar as características morfológicas dos espermatozoides de cachorros-do-mato Cerdocyon thous Linnaeus, 1766. Foram utilizados cinco animais, maiores de três anos de idade e com peso superior a 6 kg. Os animais foram anestesiados com xilazina 2%, cloridrato de ketamina 10% e atropina 1%. Foi determinado o volume testicular (testículos medidos com paquímetro). O sêmen foi coletado pelo método de eletroejaculação e a morfologia espermática analisada pelo método de preparação em câmara úmida, sendo realizado também o teste supravital (corante eosina/ nigrosina). O volume médio dos testículos foi 2,96 ± 0,56 cm3. A análise da morfologia espermática teve média de 54,2 % de defeitos totais e alta incidência de caudas dobradas e enroladas, gotas citoplasmáticas e defeitos no formato da cabeça. No teste supravital obteve-se 77,4 ± 5,17 % de células vivas. Conclui-se que os cachorros-do-mato (Cerdocyon thous) possuem volume testicular menor que outros canídeos selvagens; que a técnica de coleta de sêmen foi eficiente para a espécie e que o sêmen apresenta elevada patologia espermática, talvez devido à pequena variabilidade genética da população.


This research aimed to evaluate the testicular biometry, collect semen and analyze the morphological characteristics of the sperm of crab-eating fox Cerdocyon thous Linnaeus, 1766. Five animals older than three years-old and weighing more than 6 kg were used. The animals were anaesthetized with 2% of xylazine, 10% of ketamine hydrochloride, and 1% of atropine. The testicular volume was determined (testicles measured with caliper ruler). The semen was collected by the electroejaculation method, and the spermatozoa morphology was analyzed by the preparation in a humidified chamber method. The supravitally test was also performed (eosin /nigrosine pigment). The average volume of the testicles was 2,96 ± 0,56 cm3. Analysis of the morphology had a mean of 54.2% of total defects and high incidence of bent and rolled tails, cytoplasmic droplets and head shape defects. In supravitally tests were obtained 77.4 ± 5.17% of alive cells. We conclude that the crab-eating foxes (Cerdocyon thous) have lower testicular volume than other wild canids, that the semen collection technique was efficient for the species, and that the semen has high sperm pathology, perhaps due to the low genetic variability of the population.


Subject(s)
Animals , Semen , Cryopreservation
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